Deleted
Sample Discontinued
Experience: None
No Sequence
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Reporter

Part:BBa_K1676074:Design

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-18)


GFP Reporter with Mutant RBS Opt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers were design to contain desired mutations for PCR.


Source

Site directed mutagenesis of the oriial RBS BBa_B0034.

References